MiR 26 targeting methyltetrahydrofolate reductase inhibits the proliferation of acute myeloid leukemia cells via JAK/STAT pathway

Purpose: To investigate the effect of microribonucleic acid (miR)-26 targeting methyltetrahydrofolate reductase (MTHFR) on cell proliferation, cycle, and apoptosis in acute myeloid leukemia (AML).Methods: The expressions miR-26 three types human AML lines (HL-60, Kasumi-1, KG-1a) normal line (HS-5) were determined via quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR), while over-expression cycle cells was evaluated using counting kit-8 (CCK-8) assay flow cytometry. potential target for predicted public miRNA database TargetScan, whether binds to a dual-luciferase reporter assay. Western blotting performed determine Janus kinase (JAK)/signal transducer activator transcription (STAT) pathway-associated proteins.Results: Expression level significantly lower all than HS-5 (p < 0.05). Overexpression inhibited proliferation KG-1a cells, reduced percentage S phase, increased those G0/G1 enhanced After overexpression miR-26, protein expression levels phosphorylated (p)-JAK p-STAT down-regulated, JAK STAT did not change significantly.Conclusion: is down-regulated AML, MiR-26 MTHFR induces arrest through JAK/STAT pathway, thus inhibiting vitro.